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Version 17 (Ilian Atanassov, 02/26/2015 01:29 PM) → Version 18/29 (Ilian Atanassov, 02/26/2015 01:36 PM)
h1. Worm - RBP samples
Desired amounts:
* 100 ug protein amount for digestion
* 8-10 ug peptide amount for LC-MS/MS analysis
"X" indicates that the substance was added, "NA" that no information is available.
The excess columns show how much sample material was not used for analysis and is in a -80deg freezer.
The "Protein concentration [ug/ul]" shows the protein concentration in the aliquot from the sample preparation (also RNA measurement would be done on them)
h2. N2
|*Sample name*|*Worm culture*|*Protein concentration [ug/ul]*|*prepared by*|*RBP isolation date*|*Biological replicate*|*Is negative control*|*RBP protein yield [ug]*|*RBP protein excess[ug]*|*RBP peptide yield [ug]*|*RBP peptide excess [ug]*|*MS samples*|*MS analysis date*|*Technical replicates*|*4SU*|*XL*|*DMSO*|*Notes*|
|NA|850 T|NA|Zisong|07/2014|1|No|NA|NA|4.6|NA|140729Li_QX1_Chang_N2_1400ng_01.raw
140729Li_QX1_Chang_N2_1400ng_02.raw
140729Li_QX1_Chang_N2_1400ng_03.raw|30/07/2014|3|X|X|NA||
|1a|500 T|2|Puneet,Roman,Ilian|22/01/2015|1|No|2000|1700|32|20|NA|NA|NA|X|X|||
|1b|200 T|2|Puneet,Roman,Ilian|22/01/2015|1|No|900|720|26|13|NA|NA|NA|X|X|||
|1c|100 T|1|Puneet,Roman,Ilian|22/01/2015|1|No|300|180|16|6|NA|NA|NA|X|X|||
|1d|50 T|0.6|Puneet,Roman,Ilian|22/01/2015|1|No|100|0|17|7|NA|NA|NA|X|X|||
|2|500 T|2|Puneet,Roman,Ilian|22/01/2015|1|Yes|2000|1800|36|23|NA|NA|NA||X|X||
|3|200 T|2|Puneet,Roman,Ilian|22/01/2015|1|Yes|850|680|18|7|NA|NA|NA|X||NA|vhl-1 mutant|
h2. P6662
|*Sample name*|*Worm culture*|*Protein concentration [ug/ul]*|*prepared by*|*RBP isolation date*|*Biological replicate*|*Is negative control*|*RBP protein yield [ug]*|*RBP protein excess[ug]*|*RBP peptide yield [ug]*|*RBP peptide excess [ug]*|*MS samples name*|*MS analysis date*|*Technical replicates*|*4SU*|*XL*|*DMSO*|*Notes*|
|NA|650 T|NA|Zisong|07/2014|1|No|NA|NA|4.2|NA|140729Li_QX1_Chang_PS6662_1250ng_01.raw
140729Li_QX1_Chang_PS6662_1250ng_02.raw
140729Li_QX1_Chang_PS6662_1250ng_03.raw|31/07/2014|3|X|X|NA||
Desired amounts:
* 100 ug protein amount for digestion
* 8-10 ug peptide amount for LC-MS/MS analysis
"X" indicates that the substance was added, "NA" that no information is available.
The excess columns show how much sample material was not used for analysis and is in a -80deg freezer.
The "Protein concentration [ug/ul]" shows the protein concentration in the aliquot from the sample preparation (also RNA measurement would be done on them)
h2. N2
|*Sample name*|*Worm culture*|*Protein concentration [ug/ul]*|*prepared by*|*RBP isolation date*|*Biological replicate*|*Is negative control*|*RBP protein yield [ug]*|*RBP protein excess[ug]*|*RBP peptide yield [ug]*|*RBP peptide excess [ug]*|*MS samples*|*MS analysis date*|*Technical replicates*|*4SU*|*XL*|*DMSO*|*Notes*|
|NA|850 T|NA|Zisong|07/2014|1|No|NA|NA|4.6|NA|140729Li_QX1_Chang_N2_1400ng_01.raw
140729Li_QX1_Chang_N2_1400ng_02.raw
140729Li_QX1_Chang_N2_1400ng_03.raw|30/07/2014|3|X|X|NA||
|1a|500 T|2|Puneet,Roman,Ilian|22/01/2015|1|No|2000|1700|32|20|NA|NA|NA|X|X|||
|1b|200 T|2|Puneet,Roman,Ilian|22/01/2015|1|No|900|720|26|13|NA|NA|NA|X|X|||
|1c|100 T|1|Puneet,Roman,Ilian|22/01/2015|1|No|300|180|16|6|NA|NA|NA|X|X|||
|1d|50 T|0.6|Puneet,Roman,Ilian|22/01/2015|1|No|100|0|17|7|NA|NA|NA|X|X|||
|2|500 T|2|Puneet,Roman,Ilian|22/01/2015|1|Yes|2000|1800|36|23|NA|NA|NA||X|X||
|3|200 T|2|Puneet,Roman,Ilian|22/01/2015|1|Yes|850|680|18|7|NA|NA|NA|X||NA|vhl-1 mutant|
h2. P6662
|*Sample name*|*Worm culture*|*Protein concentration [ug/ul]*|*prepared by*|*RBP isolation date*|*Biological replicate*|*Is negative control*|*RBP protein yield [ug]*|*RBP protein excess[ug]*|*RBP peptide yield [ug]*|*RBP peptide excess [ug]*|*MS samples name*|*MS analysis date*|*Technical replicates*|*4SU*|*XL*|*DMSO*|*Notes*|
|NA|650 T|NA|Zisong|07/2014|1|No|NA|NA|4.2|NA|140729Li_QX1_Chang_PS6662_1250ng_01.raw
140729Li_QX1_Chang_PS6662_1250ng_02.raw
140729Li_QX1_Chang_PS6662_1250ng_03.raw|31/07/2014|3|X|X|NA||